Conservation genetics and demographic history of the endangered Cape Fear shiner (Notropis mekistocholas)

Saillant, E, Patton, JC, Ross, Kirstin and Gold, JR (2004) Conservation genetics and demographic history of the endangered Cape Fear shiner (Notropis mekistocholas). Molecular Ecology, 13 (10). pp. 2947-2959.

[img] PDF
Restricted to Registered users only

Download (245kB) | Request a copy


We examined allelic variation at 22 nuclear-encoded markers (21 microsatellites and one anonymous locus) and mitochondrial (mt)DNA in two geographical samples of the endangered cyprinid fish Notropis mekistocholas (Cape Fear shiner). Genetic diversity was relatively high in comparison to other endangered vertebrates, and there was no evidence of small population effects despite the low abundance reported for the species. Significant heterogeneity (following Bonferroni correction) in allele distribution at three microsatellites and in haplotype distribution in mtDNA was detected between the two localities. This heterogeneity may be due to reduced gene flow caused by a dam built in the early 1900 s. Bayesian coalescent analysis of microsatellite variation indicated that effective population size of Cape Fear shiners has declined in recent times (11-25 435 years ago, with highest posterior probabilities between 126 and 2007 years ago) by one-two orders of magnitude, consistent with the observed decline in abundance of the species. A decline in effective size was not indicated by analysis of mtDNA, where sequence polymorphism appeared to carry the signature of an older expansion phase that dated to the Pleistocene ( approximately 12 700 > 1 million years ago). Cape Fear shiners thus appear to have undergone an expansion phase following a glacial cycle but to have declined significantly in more recent times. These results suggest that rapidly evolving markers such as microsatellites may constitute a suitable tool when inferring recent demographic dynamics of populations.

Item Type: Article
Additional Information: EID:
Field of Research (2008): 06 Biological Sciences > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl. Marine Ichthyology)
06 Biological Sciences > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics
Subjects: Q Science > QH Natural history > QH426 Genetics
Date Deposited: 20 Aug 2009 16:59
Last Modified: 20 Oct 2011 03:47

Actions (login required)

View Item
View Item